Proteomics of Plant Pathogenic Fungi

نویسندگان

  • Raquel González-Fernández
  • Elena Prats
  • Jesús V. Jorrín-Novo
چکیده

Plant pathogenic fungi cause important yield losses in crops. In order to develop efficient and environmental friendly crop protection strategies, molecular studies of the fungal biological cycle, virulence factors, and interaction with its host are necessary. For that reason, several approaches have been performed using both classical genetic, cell biology, and biochemistry and the modern, holistic, and high-throughput, omic techniques. This work briefly overviews the tools available for studying Plant Pathogenic Fungi and is amply focused on MS-based Proteomics analysis, based on original papers published up to December 2009. At a methodological level, different steps in a proteomic workflow experiment are discussed. Separate sections are devoted to fungal descriptive (intracellular, subcellular, extracellular) and differential expression proteomics and interactomics. From the work published we can conclude that Proteomics, in combination with other techniques, constitutes a powerful tool for providing important information about pathogenicity and virulence factors, thus opening up new possibilities for crop disease diagnosis and crop protection.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The Role of Pathogen-Secreted Proteins in Fungal Vascular Wilt Diseases

A limited number of fungi can cause wilting disease in plants through colonization of the vascular system, the most well-known being Verticillium dahliae and Fusarium oxysporum. Like all pathogenic microorganisms, vascular wilt fungi secrete proteins during host colonization. Whole-genome sequencing and proteomics screens have identified many of these proteins, including small, usually cysteine...

متن کامل

Comparative Methods for Molecular Determination of Host-Specificity Factors in Plant-Pathogenic Fungi

Many plant-pathogenic fungi are highly host-specific. In most cases, host-specific interactions evolved at the time of speciation of the respective host plants. However, host jumps have occurred quite frequently, and still today the greatest threat for the emergence of new fungal diseases is the acquisition of infection capability of a new host by an existing plant pathogen. Understanding the m...

متن کامل

Investigation on the Effect of Anti - bacterial of the Plant Extract of Perovskia abrotanoides Karel on Number Pathogenic Bacteria and Fungi

Background: Perovskia abrotanoides is small kind of lamiaceae family that find in iran and some neighboring countries by endemic. Objective: Because The effect of anti-bacterial and anti-fungal medical plant extract on bacteria and fungi and determine the Minimal Inhibitory Concentration (MIC) has not been done so present study follow the above aim. Methodes: In this study by using 80% methan...

متن کامل

Proteomics of fungal plant pathogens: the case of Botrytis cinerea

Plant pathogenic fungi cause important yield losses in crops. In order to develop efficient and environment-friendly crop protection strategies, molecular studies of the fungal biological cycle and interaction with its host are necessary. For that reason, several approaches have been made using both classical genetic, cell biology and biochemistry and modern, holistic and high-throughput, omic ...

متن کامل

IN VITRO ANTAGONISTIC EFFECTS OF TRICHODERMA SPP. ON SEVERAL SOILBORNE PLANT PATHOGENIC FUNGI

In vitro studies with Trichoderma spp., soil-borne fungal antagonists, demonstrated that a number of isolates produced volatile and non-volatile metabolites capable of inhibiting the growth and sporulation of several soil-borne plant pathogenic fungi. Microscopic observations showed that T. harzianm and T. viride, isolated from soil samples from Ahwaz and Karaj, adversely affected the myce...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره 2010  شماره 

صفحات  -

تاریخ انتشار 2010